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Question
Biology
Posted 10 months ago
what is the order to producing recombiant insulin
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Answer from Sia
Posted 10 months ago
Answer
The order of producing recombinant insulin involves several steps including gene cloning, transformation, expression, and purification.
Solution
a
Gene cloning: The insulin gene is isolated from human DNA and inserted into a plasmid vector
b
Transformation: The recombinant plasmid is introduced into a bacterial or yeast host cell
c
Expression: The host cells are cultured to produce insulin
d
Purification: The insulin is extracted and purified from the host cells
Key Concept
Recombinant DNA technology is used to produce insulin by inserting the human insulin gene into a host cell.
Explanation
This process allows for the mass production of insulin for medical use, which is identical to the naturally occurring hormone in humans.
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