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Question
Biology
Posted 10 months ago
Why is immersion oil uses with 100X objectives
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Answer from Sia
Posted 10 months ago
Answer
Immersion oil is used with 100X objectives to increase resolution by reducing light refraction.
Solution
a
Refractive index matching: Immersion oil has a refractive index similar to glass, which reduces the refraction of light as it passes from the glass slide to the objective lens
b
Resolution enhancement: By minimizing the loss of light due to refraction, more light enters the objective lens, thereby enhancing the resolution and allowing for clearer visualization of small details
Key Concept
Refractive index matching
Explanation
Immersion oil is used to match the refractive index of the glass slide, reducing light refraction and improving resolution at high magnification.
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