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Question
Biology
Posted 9 months ago
Why doesn‘t the highly diluted antibody agglutinate the red blood cells in the wells?
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Answer from Sia
Posted 9 months ago
Answer
The highly diluted antibody does not agglutinate the red blood cells in the wells because the concentration of the antibody is too low to cross-link the antigens on the surface of the red blood cells effectively.
Solution
a
Antibody concentration: When the antibody is highly diluted, its concentration is significantly reduced. This means there are fewer antibody molecules available to bind to the antigens on the red blood cells
b
Cross-linking: For agglutination to occur, antibodies must cross-link antigens on the surface of multiple red blood cells. A high enough concentration of antibodies is required to form these cross-links
c
Insufficient binding: In a highly diluted solution, the probability of an antibody binding to antigens on different red blood cells is low, preventing the formation of the lattice structure necessary for agglutination
Key Concept
Antibody concentration and cross-linking
Explanation
A highly diluted antibody solution lacks sufficient antibody molecules to effectively cross-link antigens on the red blood cells, which is necessary for agglutination to occur.

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