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Question
Biology
Posted 10 months ago
Why doesn't a negative stain colorize the cells in the smear?
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Answer from Sia
Posted 10 months ago
Answer
Negative stains do not colorize the cells because they are repelled by the negatively charged cell surfaces.
Solution
a
Charge repulsion: The negative stain carries a negative charge which is repelled by the negatively charged components of the bacterial cell surface
b
Background staining: Instead of staining the cells, the dye stains the background, which makes the cells visible as clear areas against a dark background
Key Concept
Negative staining technique
Explanation
Negative staining relies on the repulsion between the negatively charged dye and the bacterial cell surface to leave cells unstained while coloring the background.

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