Sia
Question
Biology
Posted 10 months ago
Which protein standard curve would you use to read off the protein concentration of an unknown sample with an absorbancy value of 0.401
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BSA standard curve
BGG standard curve
Either BSA or BGG standard curves
Neither BSA or BGG standard curves
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Answer from Sia
Posted 10 months ago
A
Key Concept
Standard curves in protein quantification
Explanation
To determine the protein concentration of an unknown sample, a standard curve is used that is generated from a known protein. The BSA (bovine serum albumin) standard curve is commonly used for this purpose because BSA is a well-characterized protein that provides a reliable standard for comparison. The absorbance value of 0.401 would be compared against the BSA standard curve to determine the concentration of the unknown protein sample.
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