What prevents Cas9 cutting the genomic CRISPR array?

Question

Accepted Answer

∻Answer∻ ⚹ Cas9 does not cut the genomic CRISPR array due to the presence of anti-CRISPR proteins and the specific protospacer adjacent motifs (PAMs) that are not recognized by Cas9 in the CRISPR array. ⚹ ∻Solution∻ ‖ a ⋮ Anti-CRISPR proteins: These proteins can bind to Cas9 and inhibit its ability to cut DNA. They are part of a natural regulatory mechanism in bacteria to prevent self-targeting by the CRISPR-Cas system. ‖ ‖ b ⋮ Protospacer Adjacent Motifs (PAMs): Cas9 requires the recognition of specific PAM sequences adjacent to the target DNA sequence to initiate cutting. The genomic CRISPR array lacks these PAM sequences, thus Cas9 does not recognize or bind to these regions. ‖ ∻Key Concept∻ ⚹ Anti-CRISPR proteins and PAM sequences ⚹ ∻Explanation∻ ⚹ Anti-CRISPR proteins inhibit Cas9 activity, and the absence of PAM sequences in the genomic CRISPR array prevents Cas9 from binding and cutting the DNA. ⚹◊What role does RNA play in preventing Cas9 from cutting the genomic CRISPR array⍭ Generate me a similar question◊

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