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Question
Biology
Posted 10 months ago
Using ONE example, describe the process of active transport via a transporter.
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Answer from Sia
Posted 10 months ago
Answer
Active transport via a transporter protein involves the movement of substances against their concentration gradient using energy from ATP.
Solution
a
Example: Sodium-potassium pump (Na+/K+ ATPase) in animal cells
b
Binding of 3 Na+ ions: Three sodium ions from the cytoplasm bind to the high-affinity sites on the intracellular side of the Na+/K+ pump
c
Hydrolysis of ATP: The binding of Na+ ions stimulates the hydrolysis of ATP, which provides energy for the conformational change of the pump. One ATP molecule is hydrolyzed, releasing ADP and a phosphate group, which is temporarily attached to the pump. This process is represented by the equation: ATPADP+Pi \text{ATP} \rightarrow \text{ADP} + \text{Pi}
d
Conformational change and Na+ release: The energy from ATP hydrolysis induces a conformational change in the pump, reducing its affinity for Na+ ions and causing the release of the 3 Na+ ions outside the cell
e
Binding of 2 K+ ions: Two potassium ions from the extracellular fluid bind to the pump, which has a high affinity for K+ ions in this conformation
f
Restoration of original conformation: The binding of K+ ions triggers the release of the phosphate group, which restores the pump to its original conformation with high affinity for Na+ and low affinity for K+
g
Release of K+ ions and ready for another cycle: The K+ ions are released into the cytoplasm as the pump returns to its original conformation, and the pump is now ready to start another cycle of active transport
Key Concept
Active transport is the movement of molecules across a cell membrane from an area of lower concentration to an area of higher concentration with the use of energy from ATP.
Explanation
The sodium-potassium pump is an example of a transporter that uses ATP to move sodium and potassium ions against their respective concentration gradients, maintaining the electrochemical gradient essential for various cellular processes.

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