Explain what would happen if you performed PCR using a dNTP mixture that contained only dGTP, dATP, and dCTP?

Hint: Recall that DNA polymerase can only add nucleotides to the 3' end of a growing strand.

Question

Explain what would happen if you performed PCR using a dNTP mixture that contained only dGTP, dATP, and dCTP?

Hint: Recall that DNA polymerase can only add nucleotides to the 3' end of a growing strand.

Accepted Answer

∻Answer∻ ⚹ The PCR reaction would fail to amplify the DNA template because the absence of dTTP would prevent the synthesis of a complete complementary strand. ⚹ ∻Solution∻ ‖ a ⋮ DNA polymerase requires all four types of dNTPs to synthesize a new DNA strand. ‖ ‖ b ⋮ Without dTTP, DNA polymerase cannot add thymine nucleotides to the growing DNA strand, leading to incomplete DNA synthesis. ‖ ‖ c ⋮ Since thymine pairs with adenine, the absence of dTTP means that adenine-containing regions in the template strand cannot be properly complemented. ‖ ∻Key Concept∻ ⚹ PCR requires all four dNTPs for successful DNA amplification. ⚹ ∻Explanation∻ ⚹ In PCR, DNA polymerase synthesizes new strands by adding complementary dNTPs to the 3' end of a primer. Without dTTP, adenine in the template strand cannot be paired, halting DNA synthesis and preventing PCR amplification. ⚹◊What specific impact would the absence of dTTP in a dNTP mixture have on the PCR process in terms of DNA synthesis?⍭ Generate me a similar question◊

Sia