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Biology
Posted 9 months ago
Explain how the addition of IPTG (allolactose) results in the induction of our spider silk protein
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Answer from Sia
Posted 9 months ago
Answer
The addition of IPTG (allolactose) results in the induction of spider silk protein.
Solution
a
IPTG (Isopropyl β-D-1-thiogalactopyranoside) is a molecular mimic of allolactose, a lactose metabolite that triggers the lac operon
b
When IPTG is added to the culture, it binds to the lac repressor protein, causing a conformational change that releases the repressor from the operator region of the lac operon
c
This release allows RNA polymerase to bind to the promoter and initiate transcription of the downstream genes, including the gene encoding the spider silk protein
Key Concept
IPTG as an inducer of the lac operon
Explanation
IPTG mimics allolactose and binds to the lac repressor, freeing the operator region and allowing transcription of genes, including the spider silk protein gene.
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