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Question
Biology
Posted 10 months ago
Describe the different methods of moist heat control. Include whether they sterilize, sanitize, disinfect.
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Answer from Sia
Posted 10 months ago
Answer
Different methods of moist heat control include boiling, pasteurization, and autoclaving. Boiling and pasteurization sanitize, while autoclaving sterilizes.
Solution
a
Boiling :
Boiling water at 100°C for at least 10 minutes kills most bacteria, viruses, and fungi, effectively sanitizing the material. However, it may not kill all spores, which requires higher temperatures or longer times.
b
Pasteurization :
Pasteurization involves heating liquids to a specific temperature below boiling (typically 60-85°C) for a set period to reduce microbial load and inactivate pathogens, thus sanitizing the liquid. It does not achieve sterility as some non-pathogenic microbes may survive.
c
Autoclaving (Steam Sterilization) :
Autoclaving uses pressurized steam at temperatures above 121°C, usually for 15-20 minutes, depending on the volume. This method is capable of sterilizing as it kills all forms of microbial life, including spores.
Key Concept
Moist heat control methods vary in their temperature and time requirements, affecting their ability to sanitize or sterilize.
Explanation
Boiling and pasteurization are sanitization methods that reduce microbial load but may not eliminate all spores, while autoclaving is a sterilization method that kills all microbial life, including spores.

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