Sia
Question
Biology
Posted 9 months ago
Consider again the plates from the previous question. If our experiment is successful, then you would expect the + DNA LB/amp plate and the + DNA LB/amp/ara plate to... have different ameunts of bacterial growth on then, with the + DNA LB/amp plate having more growth. have different amounts of bacterial growth on thern, with the + DNA LB/arp/ara plate having more growth.
have roughly the same amount of growth on them.
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Answer from Sia
Posted 9 months ago
Sure, let's address the question regarding the bacterial growth on different plates.
Answer
The + DNA LB/amp/ara plate will have more growth.
Solution
a
The + DNA LB/amp plate contains ampicillin, which selects for bacteria that have taken up the plasmid with the ampicillin resistance gene
b
The + DNA LB/amp/ara plate contains both ampicillin and arabinose. Arabinose induces the expression of genes that may enhance bacterial growth
c
Therefore, the + DNA LB/amp/ara plate is expected to have more bacterial growth due to the additional presence of arabinose
Key Concept
Arabinose as an inducer
Explanation
Arabinose induces the expression of certain genes that can enhance bacterial growth, leading to more colonies on the + DNA LB/amp/ara plate compared to the + DNA LB/amp plate.
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