Assume that you set-up an experiment by seeding Human Mesenchymal Stem Cells (hMSC) on the nanofibers. You have 3 groups and 4 characterization techniques after cell seeding. Experiments should be performed 3 times for the same analysis on the same sample. The concentration of the cells is $3 imes 10^{4} \mathrm{cell} / \mathrm{cm}^{2}$ and the volume of cell suspension in each $\mathrm{NF}$ is $50 \mu \mathrm{L}$. Each nanofiber is circular with diameter of $12 \mathrm{~mm}$. For cell seeding, cell suspension was homogenized carefully mixed $100 \mu 1$ of cells with $9.9 \mathrm{ml}$ of PBS before counting. Then $50 \mu \mathrm{l}$ of cells with $50 \mu l$ of trypan blue were mixed. 75 cells were counted in 5 squares of a hemocytometer
a) Please set up an experiment by calculating ( 5 pts)
i) total sample number
ii) total sample volume
iii) cell number in each sample
iv) cell number in total volume

Question

Assume that you set-up an experiment by seeding Human Mesenchymal Stem Cells (hMSC) on the nanofibers. You have 3 groups and 4 characterization techniques after cell seeding. Experiments should be performed 3 times for the same analysis on the same sample. The concentration of the cells is $3 	imes 10^{4} \mathrm{cell} / \mathrm{cm}^{2}$ and the volume of cell suspension in each $\mathrm{NF}$ is $50 \mu \mathrm{L}$. Each nanofiber is circular with diameter of $12 \mathrm{~mm}$. For cell seeding, cell suspension was homogenized carefully mixed $100 \mu 1$ of cells with $9.9 \mathrm{ml}$ of PBS before counting. Then $50 \mu \mathrm{l}$ of cells with $50 \mu l$ of trypan blue were mixed. 75 cells were counted in 5 squares of a hemocytometer
a) Please set up an experiment by calculating ( 5 pts)
i) total sample number
ii) total sample volume
iii) cell number in each sample
iv) cell number in total volume

Accepted Answer

∻Answer∻ ⚹ i) Total sample number: 36 samples ii) Total sample volume: 1.8 mL iii) Cell number in each sample: 1,800 cells iv) Cell number in total volume: 64,800 cells ⚹ ∻Solution∻ ‖ a ⋮ To calculate the total sample number, we consider the number of groups, the number of characterization techniques, and the number of repetitions. The formula is: Total sample number = Number of groups × Number of techniques × Number of repetitions. ‖ ‖ b ⋮ The total sample volume is calculated by multiplying the volume of cell suspension used for each nanofiber by the total number of samples. The formula is: Total sample volume = Volume per NF × Total sample number. ‖ ‖ c ⋮ To find the cell number in each sample, we use the given cell concentration and the area of the nanofiber. The area of a circle is calculated by $A = \pi r^2$, where $r$ is the radius. The cell number in each sample is then: Cell number per sample = Cell concentration × Area of NF. ‖ ‖ d ⋮ The cell number in the total volume is the cell number in each sample multiplied by the total sample number. The formula is: Cell number in total volume = Cell number per sample × Total sample number. ‖ ∻Key Concept∻ ⚹ Experimental design and calculation of sample sizes and volumes in cell culture experiments ⚹ ∻Explanation∻ ⚹ The student's question involves setting up an experiment with hMSCs on nanofibers, requiring calculations for sample numbers, volumes, and cell counts based on given parameters and cell culture practices. ⚹◊What is the formula to calculate the total sample number in the experiment involving seeding hMSC on nanofibers?⍭ Generate me a similar question◊

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